When analyzing the distribution of polysaccharide molecular weight using dynamic light scattering (DLS), is it necessary to use standards?
When using dynamic light scattering (DLS) to analyze the molecular weight distribution of polysaccharides, it is generally not necessary to use standards. DLS determines the particle size distribution and molecular weight by measuring the Brownian motion of particles or molecules in solution. It calculates the diffusion coefficient from the intensity fluctuations of scattered light and then determines the particle size based on the Stokes-Einstein equation.
Although DLS does not directly rely on standards to measure molecular weight distribution, using standards in the following situations can enhance the accuracy and reliability of the analysis:
1. Instrument Calibration: Before DLS analysis, using standard particles with known sizes can calibrate the instrument to ensure accurate measurement results.
2. Data Comparison: Standards can be used to compare sample data to ensure the validity of measurement results, especially when comparing different samples.
In conclusion, while standards are not mandatory for DLS analysis, they aid in improving the accuracy and reliability of the analysis in terms of instrument calibration and data comparison.
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