Can Escherichia coli samples, after being quickly frozen in liquid nitrogen and stored at -80 degrees for two weeks, still be sent for transcriptomics and metabolomics testing?
E. coli samples that have been rapidly frozen with liquid nitrogen and stored at -80°C for two weeks are typically suitable for transcriptomics and metabolomics analysis. Rapid freezing with liquid nitrogen effectively preserves the biological activity of cells, while -80°C storage slows down degradation processes. However, attention should still be paid to the following points:
1. Sample Integrity: Ensure that the sample does not thaw during handling and transportation. If the sample thaws during transport, it may affect the analysis results.
2. RNA Quality: Before conducting transcriptomics analysis, it is best to perform RNA quality assessment to confirm RNA integrity (usually evaluated using RIN values).
3. Metabolomics Stability: Some metabolites may degrade or transform during long-term storage, so efforts should be made to minimize these effects before sample processing.
It is recommended to consult with relevant technical support before conducting specific analyses in the laboratory to ensure optimal sample handling and analysis conditions.
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