Can mass spectrometry be used to detect the content of a certain sample in animal tissues? How should the tissue be processed?
Mass spectrometry technology can be used to detect the content of certain samples (such as proteins, lipids, metabolites, etc.) in animal tissues. The treatment of animal tissue samples typically involves the following steps:
1. Sample Collection and Preservation
Animal tissues should be frozen as soon as possible after collection (-80°C) to prevent the degradation of molecules within the samples. If necessary, rapid freezing with liquid nitrogen can be used to ensure sample stability.
2. Tissue Lysis and Homogenization
(1) Use a homogenizer (such as a glass homogenizer or ultrasonic disruptor) to mechanically or sonically treat the tissue, breaking cell membranes and releasing intracellular components.
(2) For lipid-soluble substances or proteins, appropriate buffers can be added to ensure complete release of cellular components.
3. Removal of Macromolecules and Impurities
(1) Depending on the type of molecule to be analyzed, centrifugation is usually needed to remove cell debris, nucleic acids, fats, and other macromolecular impurities.
(2) For metabolite or small molecule analysis, solid-phase extraction (SPE) or liquid-liquid extraction may be required to further purify the sample.
4. Extraction of Target Compounds
(1) If analyzing small molecule metabolites, organic solvents (such as methanol, acetonitrile, chloroform, etc.) can be used for extraction. For certain analyses, methods specific to the target molecule can be chosen.
(2) For proteins or peptides, appropriate lysis buffers (such as solutions containing urea or thiourea) are typically needed for protein lysis.
5. Sample Concentration and Drying
For some analyses, the extracted solution may need to be concentrated using vacuum centrifugation to remove solvents or partially evaporate the solvent to enhance detection sensitivity.
6. Re-dissolution and Dilution
Dissolve the sample in a solvent suitable for mass spectrometry analysis (such as water, acetonitrile, methanol, etc.) and adjust the sample's concentration and pH according to the mass spectrometer's requirements.
7. Mass Spectrometry Analysis
Finally, the processed sample is sent to the mass spectrometer for analysis, and the appropriate chromatographic separation method is selected based on the type of mass spectrometry (such as LC-MS, GC-MS, MALDI-MS, etc.).
Biotechpack uses Thermo Fisher's Q Exactive HF mass spectrometry platform, Orbitrap Fusion mass spectrometry platform, and Orbitrap Fusion Lumos mass spectrometry platform combined with Nano-LC systems to provide comprehensive analysis services for proteins, metabolites, lipids, and other substances. You only need to inform us of your experimental goals and send us your samples, and we will handle all subsequent matters, including sample extraction, purification, concentration, mass spectrometry analysis, raw data analysis, and bioinformatics analysis. Feel free to consult us for more details!
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