Cellular Metabolomics, during the final step of discarding the supernatant, mistakenly centrifuged at 7000g for 3 minutes. Can this sample still be sent for metabolomics?
If during cell metabolomics sample processing, 7000g centrifugation for 3 minutes was mistakenly used, it may lead to cell rupture, metabolite release, or sample contamination, thereby affecting analysis results. The specific impact depends on the extent of cell rupture and sample contamination.
Recommendation:
1. Check the clarity of the supernatant: If the supernatant remains clear and no visible precipitate or suspended particles are present, the sample may be minimally affected and can proceed for analysis.
2. Repeat supernatant collection: If conditions allow, the sample can be centrifuged again at a lower force (e.g., 500-1000g) for a short time (1-2 minutes), then discard any remaining fine precipitate to improve supernatant purity.
3. Tolerance of the analytical requirement: Some metabolomics analyses (e.g., mass spectrometry) have high requirements for sample purity. If residual proteins or macromolecules might affect mass spectrometry or other high-sensitivity instruments, it is advisable to confirm with the analytical institution whether the sample meets the requirements.
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