Mass Spectrometry Analysis of Protein Conjugates
Protein adducts are complexes formed by the binding of a chemical substance with a protein. Mass spectrometry analysis of protein adducts aids in measuring the body's exposure to foreign substances. Biotage provides protein adduct analysis services based on mass spectrometry technology.
Protein adducts
In biology, an adduct refers to a complex formed by the binding of a compound with a biomolecule (such as DNA or protein). Adducts contain all atoms of their components and are considered a unique molecular species. Protein adducts are covalent modifications caused by the reaction between electrophilic reagents and nucleophilic sites in proteins (e.g., N-terminus or amino acid side chains containing thiol or amine functional groups). In toxicology, the measurement of protein adducts can serve as a method to assess the body's exposure to foreign substances.
Mass spectrometry analysis of protein adducts
Mass spectrometry analysis of protein adducts refers to the analytical measurement of protein adducts using mass spectrometry technology. Since the early measurements of protein adducts, mass spectrometry has been the preferred analytical detection technique because it provides qualitative and quantitative information about modified proteins. In toxicology research, mass spectrometry analysis of protein adducts primarily measures adducts of expected reactive metabolites of toxic chemicals with a given protein. Mass spectrometry analysis samples are mainly measured by determining the relative molecular mass. The analysis and detection of protein adducts are also achieved by analyzing the relative molecular mass of peptide segments or fragment ions. Generally, mass spectrometry is combined with chromatography for the analysis and detection of protein adducts.
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