In protein circular dichroism analysis, how should the spectrum be analyzed?

When analyzing protein CD spectra, the following aspects are usually considered:

1. Peak Shape Identification:

Firstly, observe the shape of the CD spectrum peaks. The alpha-helix structure of proteins shows negative circular dichroism peaks at wavelengths around 208 and 222 nm, while beta-sheet structures exhibit a positive peak and a negative peak around 218 nm.

2. Peak Intensity Comparison:

Comparing the intensity of peaks can estimate the relative content of various secondary structures. For example, stronger negative peaks usually indicate a higher content of alpha-helix structures.

3.Quantitative Analysis:

• Specialized software (such as DichroWeb, SELCON, CONTIN, etc.) can be used to analyze spectral data to estimate the proportions of alpha-helix, beta-sheet, beta-turn, and random coil structures.
• These software tools infer the secondary structure composition of samples by comparing experimental data with standard datasets of known structures.

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