What is the determination of amino acid sequences by mass spectrometry

Mass spectrometry for determining amino acid sequences is a method that uses a mass spectrometer to determine the sequence of amino acids in proteins or peptides.

I. Method Principle

First, the protein sample is ionized and then accelerated in an electric and magnetic field. These ions travel at different speeds through the flight tube, with smaller ions traveling faster than larger ones. By measuring the time it takes for these ions to reach the detector, the mass-to-charge ratio of the ions can be determined. Then, by comparing this ratio with the known mass-to-charge ratios of amino acid ions, the amino acid sequence of the peptide fragment can be determined.

II. Reagents and Equipment

1. Mass Spectrometer

The main tool for mass spectrometry, capable of measuring the mass and charge of ions.

2. Ion Source

Part of the mass spectrometer used to ionize protein or peptide molecules.

3. Analyzer Tube

Where ions travel. Depending on the mass and charge of the ions, they travel at different speeds.

4. Detector

Used to measure the time it takes for ions to reach it, thus allowing the calculation of the mass-to-charge ratio of the ions.

5. Protease

Used to break down proteins into smaller peptide fragments for mass spectrometry analysis.

III. Applications

Mass spectrometry for determining amino acid sequences is widely used in proteomics research, including protein expression profiling, protein modification studies, and protein interaction research. Additionally, it is used in disease diagnosis, drug development, and biotechnology.

BiotechPack, A Biopharmaceutical Characterization and Multi-Omics Mass Spectrometry (MS) Services Provider

Related Services:

Protein Sequencing

Protein N/C Terminal Sequencing

Biopharmaceutical N/C Terminal Sequencing

Protein N-terminal Sequencing Based on Edman Degradation