HPLC Peptide Purity Analysis
High-performance liquid chromatography (HPLC) is a modern chromatographic separation technique widely used in biological, chemical, and medical research..In peptide purity analysis, HPLC is mainly used to detect and identify the composition and content of peptides in samples, toevaluate and detect the purity and impurities in peptide samples.The principle isto dissolve the peptide to be tested in the mobile phase and separate it through the chromatographic column. The column is packed with a stationary phase that relies on different interaction mechanisms to produce different retention times for components in the peptide sample. Through the detector, qualitative and quantitative analysis of peptidecomponents in the sample is achieved, identifying peptide purity.
Biotech PackBiotechnology HPLCPeptide Purity AnalysisProcess
1. Sample Preparation:
Dissolve the peptide sample in an appropriate solvent (such as water, acetonitrile, or methanol) to prepare the test solution.
2. Set HPLC Conditions:
Set appropriate parameters for the mobile phase, flow rate, gradient program, and detection wavelength based on the sample characteristics and the selected column type.
3. System Equilibration:
Pass the mobile phase through the column to reach equilibrium, ensuring stable and reproducible analytical results.
4. Sample Injection:
Inject the test solution into the chromatographic system using an auto-sampler or manual injection device.
5. Separation Process:
The peptide sample enters the column carried by the mobile phase and is separated based on the interactions between the stationary phase and the peptides.
6. Detection and Data Collection:
The separated peptide components sequentially pass through the detector, generating signals. The instrument records retention time and signal intensity, producing a chromatogram.
7. Data Processing and Analysis:
Analyze the chromatogram using specialized software to qualitatively and quantitatively assess peptide purity based on retention time and signal intensity.
HPLCMain Applications of Peptide Purity Analysis
1. Drug Development and Preparation:
Analyzepeptidedrugsfor purity, composition, impurities, and content,ensuring the purity and impurity content of peptidedrugsto ensure drug quality and safety,achieving high-purity peptide drug preparation,providing data support for peptide drug development.
2. Industrial Production:
Monitor purification steps, degradation, and impurity generation during peptide production, providing a basis for process optimization and quality control.
3. Clinical Diagnosis:
Determine peptide levels in biological samples (such as serum, urine) to provide a basis for clinical diagnosis, disease monitoring, and treatment evaluation.
4. Food Safety:
Detect the purity of peptide additives and residues in food to ensure safety and quality.
5. Environmental Monitoring:
Detect peptide pollutants in environmental samples (such as water, soil) to provide data support for environmental risk assessment and pollution control.
About Samples
|
Sample Type |
Sample Quantity |
|
|
Synthetic Peptides (Pharmaceutical peptides, research peptides, labeled peptides, etc.)
|
Less than10kDa |
1-5mg |
|
10-50kDa |
5-10mg |
|
|
Greater than50kDa |
10-20mg |
|
|
Natural Extracts (Animal and plant peptides, microbial peptides, etc.)
|
Less than10kDa |
5-10mg |
|
10-50kDa |
10-20mg |
|
|
Greater than50kDa |
20-50mg |
|
|
Protein Digests (Enzyme fragments, modified peptides, etc.)
|
Less than10kDa |
5-10mg |
|
10-50kDa |
10-20mg |
|
|
Greater than50kDa |
20-50mg |
|
Bilingual Project Report
In the technical report, Biotech Pack will provide you with a detailed bilingual (Chinese and English) technical report, including:
1. Experimental Procedures (Chinese and English)
2. RelatedChromatographicParameters (Chinese and English)
3. HPLCDetailed Information on Peptide Purity Analysis
4. ChromatographicImages
5. Raw Data
HPLCOne-Stop Service for Peptide Purity Analysis
You only need to place an order and send the samples
Biotech Pack provides a one-stop service: sample processing, machine analysis, data analysis, and project report
Related Services
Characterization of Protein Purity and Homogeneity
SDS-PAGE Protein Purity Analysis
Protein Purity Analysis (Size Exclusion/Reverse Phase Chromatography)
Peptide Mass Spectrometry Identification
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