When using HPLC to analyze monosaccharide composition, it was found that there is another peak right after fucose.
In HPLC analysis of monosaccharide composition, if an unknown peak appears immediately after the fucose peak, it may be other monosaccharides or some derivatives. Common monosaccharides include:
- Galactose: It may appear close to fucose under many analytical conditions, but usually at a different retention time.
- Mannose: It may sometimes appear near fucose.
- Glucose: Although it usually appears at a different retention time, under certain conditions it may be close to fucose.
To accurately identify this unknown peak, consider the following methods:
- Standard comparison: Use standards of known monosaccharides to compare retention times.
- Mass spectrometry analysis: Determine the molecular weight and structural information of the peak through mass spectrometry (MS).
- Change chromatographic conditions: Try changing the mobile phase or column conditions to separate the unknown peak.
Specific sugar identification needs to be judged in combination with the specific situation of the known sample and analytical conditions. If possible, it is recommended to use a combination of the above methods to improve identification accuracy.
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