Peptide Mass Spectrometry Analysis Principle
The principle of peptide mass spectrometry analysis is based on the Shotgun method: proteins are enzymatically digested into small peptide mixtures, which are then separated and purified by high-performance liquid chromatography (HPLC) before being introduced into high-resolution mass spectrometers for analysis. The peptides are ionized in the mass spectrometer and acquire a certain amount of charge. By analyzing with a detector, the mass-to-charge ratio (m/z) of the peptides can be obtained, allowing for the calculation of the relative molecular mass of the peptides.
Peptide mass spectrometry analysis involves selecting peptides with a certain mass-to-charge ratio (m/z) through primary mass spectrometry to enter secondary mass spectrometry for fragmentation. By comparing the secondary mass spectrometry information with the relevant database, the precise sequence of the peptides can be obtained, thus achieving peptide analysis. Techniques such as MALDI-TOF MS or NanoLC-MC/MS are commonly used for peptide analysis, among which NanoLC-MC/MS offers higher detection sensitivity and reliability.
Biotech company Biotage uses Thermo Fisher's Orbitrap Fusion Lumos mass spectrometry platform combined with nanoLC-MS/MS nanoliter chromatography technology to providepeptide mass spectrometry identificationservices. This service can detect the sequence information of peptides, thereby obtaining the sequence or post-translational modification information of peptides. You only need to inform us of your experimental purpose and send the samples; we will handle all subsequent matters of the project, including cell culture, protein extraction, protein purification, protein digestion, peptide separation, mass spectrometry analysis, raw mass spectrometry data analysis, and bioinformatics analysis.
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