Mass Spectrometry and Edman Degradation N-terminal Sequencing
Almost all protein synthesis begins at the N-terminus, and the amino acid sequence composition plays an important role in the overall biological function of the protein. Therefore, analysis of the protein's N-terminal sequence is crucial for research in biomedicine and other fields.
The two main methods for protein N-terminal sequencing are Edman degradation and mass spectrometry. Mass spectrometry digests proteins into peptides consisting of 5-25 amino acids, which are then analyzed by LC-MS/MS. The collected data is matched with theoretical sequence databases to confirm the N-terminal sequence. Mass spectrometry can detect blocked and modified protein N-termini and has become the preferred method for full-length antibody sequencing.
Unlike mass spectrometry, Edman degradation is a chemical method, and the resulting Edman sequencing has become a mature protein sequencing technique. It does not require protein databases for direct sequence analysis. A key advantage of Edman sequencing is its intuitive results, allowing direct acquisition of amino acid sequences. However, it cannot be used for N-terminal blocked or modified amino acid residues. The quality of Edman sequencing decreases as the number of detected amino acids increases, so it is usually used to determine the first 30-60 amino acids, with a maximum detection length of no more than 100 amino acids. Therefore, Edman sequencing cannot be used for full-length protein sequencing.
Mass spectrometry and Edman degradation each have their strengths and complement each other. Therefore, combining LC-MS/MS N-terminal sequencing technology with Edman degradation-based sequencing allows for accurate identification of protein N-terminal sequences.
BaiTai Park BioTech utilizes Shimadzu's Edman sequencing system to provideEdman degradation-based protein N-terminal sequencingservices for researchers and clients. Our sequencing system can determine the sequence information of the first 30 N-terminal amino acids. With a specific protein sample loading system, it can determine 60-70 N-terminal amino acids. BaiTai Park BioTech has also established a platform for N-terminal sequencing using advanced LC-MS/MS technology, capable of detecting blocked and modified protein termini, whichcomplements Edman degradation-based protein N-terminal sequencingto ensure smooth N-terminal sequencing services.
Related services:
Protein N/C-terminal sequencing
Biopharmaceutical N/C-terminal sequencing
Full protein sequence determination
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