Differences between Edman protein sequencing and mass spectrometry

Protein sequencing refers to determining the amino acid composition and arrangement of proteins, also known as primary structure analysis. Currently, methods for protein amino acid sequence analysis can be broadly divided into mass spectrometry-based methods and non-mass spectrometry methods. Edman degradation is the most commonly used non-mass spectrometry method. Mass spectrometry and Edman degradation achieve protein sequence analysis based on different principles, and the fragments they can analyze are also different. Edman degradation is a sequencing method for the N-terminal sequence, but it is ineffective for proteins with blocked N-terminals. Mass spectrometry can be used for de novo protein sequencing, full protein sequence analysis, and protein terminal (N/C) sequencing, including proteins with blocked N-terminals. In short, the main difference between the two lies in the principles of analysis and the sequences they can analyze.

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