How much protein is needed for Edman protein sequencing?
In Edman protein sequencing, the amount of protein required typically depends on various factors such as the sensitivity of the sequencing equipment, the purity of the protein, and the complexity of the sequence. Traditionally, Edman degradation is used to determine the amino acid sequence of proteins or peptides and generally requires about 1 to 100 picomoles (pmol) of protein sample. This corresponds to approximately 0.1 to 10 micrograms (μg) of protein, depending on the molecular weight of the protein.
For conventional Edman sequencing, typically about 10-100 picomoles (pmol) of protein sample is needed. This translates to several hundred nanograms to a few micrograms of protein mass. However, with modern, high-sensitivity equipment, this amount can be even less, reaching below the single picomole level.
Figure 1. Edman Sequencing Experimental Workflow Diagram
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